Issue 01 — Reconstitution Without the Guesswork

Welcome to the first issue of The WikiPeps Letter. We started WikiPeps for one reason: there is a lot of peptide information online, and most of it is either hype, sales copy, or guesswork. We don't sell peptides. We teach — from the community, to the community. Everything here is educational, never medical advice, and never a nudge to obtain or use anything. With that said, let's talk about the step that trips up more people than any other: reconstitution.

The fear is the math, not the mixing#

When people picture reconstituting a lyophilized peptide, they imagine the physical act — adding water to a vial. But the part that actually causes anxiety is the arithmetic: "How much water do I add? How many units is that? Did I just measure ten times too much?" The good news is that all of it reduces to a single relationship: concentration = peptide amount ÷ diluent volume. Once you know your concentration, every other number falls out of it.

Here is the whole thing in one worked example. Say you have a 5 mg vial and you add 2 mL of bacteriostatic water. Convert milligrams to micrograms first (5 mg = 5,000 mcg), divide by your water (5,000 ÷ 2 = 2,500 mcg/mL), then divide by 100 because a U-100 insulin syringe has 100 units per mL (2,500 ÷ 100 = 25 mcg per unit). That's it. Now any amount you want to measure is just "desired mcg ÷ 25." We break this down with a full table in our mixing-math guide.

The one habit that protects your peptide#

If you remember nothing else from this issue, remember this: do not shake the vial. Peptides are fragile molecules, and the shear forces and foaming from vigorous shaking can degrade them. Add the water slowly down the inside glass wall, then swirl or roll gently until the solution is clear. It feels almost too gentle. That's the point. Our reconstitution guide walks through every step.

Next week#

We'll get into reading an insulin syringe — what a "unit" actually is, why it's a measure of volume and not of peptide, and how that one misunderstanding leads to the scariest dosing mistakes. As always: this is education, and a licensed clinician is the right person for any decision about your health. See you in Issue 02.